Puerarin reduces ethanol-induced-apoptosis of spermatogenic cells in rat testis / 基础医学与临床
Basic & Clinical Medicine
; (12): 1291-1295, 2009.
Article
de Zh
| WPRIM
| ID: wpr-440649
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WPRO
ABSTRACT
Objective To investigate potential protection by protect against ethanol-induced apoptosis of spermato-genie cells in rat testis. Methods Thirty SD adult male rats were randomly divided into three groups: normal group, alcohol group and puerarin group. At 40th day, BCL-2 and BAX of spermatogenic cells of testis tissue were checked by RT-PCR and immunohistochemistry; Apoptosis of spermatogenic cells was determined by TUNEL. Re-suits The results of RT-PCR and immunohistochemistry indicated that BCL-2 and BAX of spermatogenie cells were not significanty different between puerarin group and normal group, but there was the significant difference between alcohol group and puerarin group (P <0.01). Apoptosis of spermatogenic cells in alcohol group was significantly higher than normal group. Conclusion Spermatogenic cells could generate apoptosis by changing the expression of BCL-2 and BAX. Puerarin could inhibit this damage of didymus by alcohol.
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WPRIM
langue:
Zh
Texte intégral:
Basic & Clinical Medicine
Année:
2009
Type:
Article