Galectin-3 Expression and BRAF Mutation in Cases of Cytologically Suspicious Papillary Thyroid Carcinoma
Korean Journal of Pathology
; : 191-198, 2010.
Article
Dans Ko
| WPRIM
| ID: wpr-48171
Responsable en Bibliothèque :
WPRO
ABSTRACT
BACKGROUND:
Fine needle aspiration, which is known as the most accurate and cost-effective method for diagnosis of thyroid nodule, still may result in indeterminate cases that are pauci-cellular and show minor nuclear atypia, but most cases are associated with suspicion of papillary thyroid carcinoma (PTC). A B-type Raf kinase (BRAF) mutation was found in about half of PTCs and galectin-3 was expressed by malignant tumors, helping us to differentiate malignancies from benign lesions.METHODS:
Cases studied included histologically 44 confirmed PTC cases and 18 benign cases previously diagnosed as suspicious of PTC using cytologic examination. Cases were analyzed for galectin-3 expression by immunohistochemical staining and BRAF mutation by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with a new restriction enzyme.RESULTS:
All 44 cases of PTC and 8 of 18 benign controls expressed galectin-3. BRAF mutations were found in only 9 of the 44 PTC cases. Assessment of galectin-3 expression demonstrated high sensitivity but low specificity. Evaluation of BRAF mutation revealed high specificity and low sensitivity.CONCLUSIONS:
This study suggests that the combined application of these two methods for PTC of suspicious cytology is complementary.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phosphotransferases
/
Glande thyroide
/
Facteur IX
/
Tumeurs de la thyroïde
/
Carcinomes
/
Sensibilité et spécificité
/
Nodule thyroïdien
/
Galectine -3
/
Cytoponction
Type d'étude:
Diagnostic_studies
langue:
Ko
Texte intégral:
Korean Journal of Pathology
Année:
2010
Type:
Article