Expression of progesterone receptor a protein in prokaryotic and preparation of monoclonal antibodies to PR-A / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 1013-1016, 2016.
Article
de Zh
| WPRIM
| ID: wpr-496532
Bibliothèque responsable:
WPRO
ABSTRACT
Objective:To prepare for mAb of progesterone receptor. It would provide support for the immunohistochemistry behind. Methods:Target gene connected together with a carrier by seamless cloning method. The target protein that expression by inducing was collected. And with cell fusion method , the monoclonal antibodies were preparation. Then the mAb were detected by IHC. Results: The mAb ( clone 7C7 ) was detected and it found positive for the breast, uterine fibroid tissue, showed negative in colorectal cancer tissue, smooth muscle tissue, the goal of the claim were achieve. Conclusion: Finally, we found the method that prepare for mAb was far beyond our imagination. The result of IHC on different samples about mAb(7C7)obtained compliance with an-ticipation. Study on the difference between the PR-A and PR-B had significance.
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Immunology
Année:
2016
Type:
Article