LC-MS based metabolomics studies on natural menopause(aging)in a rat model / 国际药学研究杂志
Journal of International Pharmaceutical Research
; (6): 711-717, 2016.
Article
de Zh
| WPRIM
| ID: wpr-498173
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To further understand the natural metabolic characteristics of menopausal syndrome,and explore the role of biological pathways. Methods Our experiment adopted the HPLC-MS/MS technology of metabolomics researching methods, aiming at the comprehensive change characteristics in metabolism group level of menopausal rat and searching for age-related biomark?ers. The urine of 6 and 18 months rat was taken for HPLC-MS/MS metabolomics analysis. Besides,XCMS online analysis was used for positive and negative ion detection spectrum and the principal component analysis was used to identify biomarkers. Finally ,MetPA was used to implement a Google-map style interactive visualization system,which has analyzed the metabolic pathway. Pathway views were generated dynamically based on interactions with the visualized system. Results The TCA cycle,arginine and proline metabo?lism and aldehyde acid metabolism are related to aging rat metabolite pathways. Conclusion Age-related metabolic pathways are mainly related to the respiratory chain reaction and the urea cycle,which are influenced by liver mitochondrial function.
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Prognostic_studies
langue:
Zh
Texte intégral:
Journal of International Pharmaceutical Research
Année:
2016
Type:
Article