Roles of PKC and ERK in the mechanism of delayed protection of anoxia preconditioning in rat cardiac myocytes / 中国病理生理杂志

ABSTRACT

AIM: To investigate the roles of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK) in delayed protection mechanism of anoxia preconditioning (APC) in rat cardiomyocytes.METHODS: The anoxia/reoxygention (A/R) injury, APC and PMA (an activator of PKC) preconditioning models were established in cultured neonatal rat cardiomyocytes and the effects of PKC and ERK blokers on the models were observed. ERK activity was assayed at 10 min after preconditioning in every group. The cellular MDA, SOD, cell viability and LDH release were measured at the end of the study. RESULTS: Compared with the cardiomyocyte in A/R group, the percentage of viable cells and SOD activity were greatly increased (P