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Effect of Arsenic Trioxide on Human Hepatoma Cell Proliferation and Its uPAR Expression / 中国药房
China Pharmacy ; (12)2007.
Article de Zh | WPRIM | ID: wpr-532959
Bibliothèque responsable: WPRO
ABSTRACT
OBJECTIVE:To investigate the effect of arsenic trioxide(As2O3)on human hepatoma cells SMMC-7721 viability and the expression of urokinase plasminogen activator receptor(uPAR).METHODS:SMMC-7721 cells were cultured and treated with As2O3 at different concentrations for different time with untreated SMMC-7721 cells(without As2O3 treatment)served as control.The proliferation rate of the SMMC-7721 cells was measured by MTT assay;the change of cell cycle was detected by flow cytometry analysis;the effect of As2O3 on uPAR mRNA levels was analyzed by real-time fluorescent quantitative polymerase chain reaction.RESULTS:As2O3 had significant inhibitory effect on SMMC-7721 proliferation in time-dependent and concentration-dependent manner.As compared with control group,As2O3 significantly decreased the proportion of cells in G0/G1 phase but increased the cell proportion in G2/M phase,and it markedly down-regulated the level of uPAR mRNA expression(P
Mots clés
Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: China Pharmacy Année: 2007 Type: Article
Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: China Pharmacy Année: 2007 Type: Article