Investigation on the relationship among follicular development and insulin-like growth factor-Ⅰ and its receptor mRNA during ovulation stimulation period / 中华内分泌代谢杂志

ABSTRACT

Objective To observe the relationship among follicular development and insulin-like growth factor Ⅰ (IGF-Ⅰ) and IGF-Ⅰ receptor (IGF-ⅠR) mRNA during ovulation stimulation period in an in-vitro fertilization (IVF) program. Methods IGF-Ⅰ levels in serum samples obtained before (menstrual day 3) and after stimulated (at the time of ovum pick-up) and follicular fluid samples were measured by ELISA. The expression of IGF-Ⅰ mRNA in the granulosa cell obtained from follicular aspiration was determined by RT-PCR. Results There was no significant difference between IGF-Ⅰ levels of serum samples before or after stimulated and follicular fluid. In patients with less than 4 follicls (diameter≥14 mm) at the time of ovum pick-up the IGF-Ⅰ mRNA level was significantly lower than that in patients with more than 4 follicles. Conclusion The serum IGF-Ⅰ levels fail to predict the numbers of follicular development; the levels of IGF-ⅠR mRNA in granulosa cells forecast the responsiveness of ovary to the stimulation of gonadotropins in an IVF program.