Prognostic Implication of Semi-quantitative Immunohistochemical Assessment of CD20 Expression in Diffuse Large B-Cell Lymphoma
Journal of Pathology and Translational Medicine
; : 96-103, 2016.
Article
de En
| WPRIM
| ID: wpr-56491
Bibliothèque responsable:
WPRO
ABSTRACT
BACKGROUND: Immunohistochemical demonstration of CD20 in diffuse large B-cell lymphoma (DLBCL) is prerequisite not only for the diagnosis but also for assigning patients to rituximab-containing chemotherapy. However, little is known about the impact of abundance of CD20 expression assessed by immunohistochemistry on the clinical outcome of DLBCL. We performed a semi-quantitative immunohistochemical analysis of CD20 expression in DLBCL to examine the prognostic implication of the level of CD20 expression. METHODS: Pre-treatment diagnostic tissue samples from 48 DLBCL patients who were treated with rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) regimen were represented in a tissue microarray and immunostained for CD20. The relative abundance of CD20 expression was semi-quantitatively scored using a web-based ImmunoMembrane plug-in. Receiver operating characteristic curve analysis was used to determine a prognostically relevant cut-off score in order to dichotomize the patients into CD20-high versus CD20-low groups. RESULTS: The levels of CD20 expression were heterogeneous among the patients, with a wide and linear distribution of scores. Patients in CD20-low group showed significantly poor clinical outcome. CONCLUSIONS: The levels of CD20 expression in DLBCL are heterogeneous among the patients with DLBCL. A subgroup of the patients with CD20 expression levels below the cut-off score showed poor clinical outcome.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Vincristine
/
Prednisone
/
Immunohistochimie
/
Lymphocytes B
/
Doxorubicine
/
Courbe ROC
/
Lymphome B
/
Antigènes CD20
/
Cyclophosphamide
/
Analyse sur puce à tissus
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites du sujet:
Humans
langue:
En
Texte intégral:
Journal of Pathology and Translational Medicine
Année:
2016
Type:
Article