Effect of subculture at logarithmic phase on kinetics of cell cultures of Taxus chinensis var. mairei / 中草药

ABSTRACT

Object To solve the problem of low growth rate an d metabolism level in Taxus chinensis var. mairei (Lem?e et L?vl.) Cheng et L. K. Fu Methods Cells on logar it hmic phase (20 d) and stationary phase (30 d) were subcultured individually. Th euptake of carbohydrate, nitrogen and phosphate from the medium was examined, th e growth rate of Taxus cell and the synthesis of taxol were anal y zed too during the whole growth period Results The Taxus cell subcultured at logarithmic phase uptook carbohydrate and nit rate earlier than that subcu ltured at stationary phase. The special growth rate of the former was 1.5 folds of that of the latter. The production rate of taxol was increased by nearly 4 ti m es Conclusion Subculturing at logarithmic phase will benefi t the biomass accumulation and the taxol synthesis