Effects of methylprednisolone on pneumocyte apoptosis during pulmonary ischemia/reperfusion injury in rats / 医学研究生学报
Journal of Medical Postgraduates
; (12)2003.
Article
de Zh
| WPRIM
| ID: wpr-589121
Bibliothèque responsable:
WPRO
ABSTRACT
Objective:To investigate the effects of methylprednisolone(MP)on pneumocyte apoptosis during lung ischemia/reperfusion injury in rats and to study the possible role of MP in pneumocyte apoptosis.Methods:Forty-two male Sprague-Dawley rats used for unilateral lung ischemia/reperfusion model were randomly divided into three groups:sham operation group(Sh group),ischemia/reperfusion group(I/R group),and methylprednisolone group(MP group).Each group has two subgroups of three hours and six hours.Apoptosis rate in lung tissue was detected by the way of Annexin-V-PI in flow cytometer.Expression of I?B-? in lung was observed by immunohistochemical stain.The index of quantitative assessment of histological lung injury(IQA),the wet to dry weight ratio(W/D),the pathological and ultrastructure changes of lung tissue were measured.Results:Apoptosis rate,W/D,IQA of lung tissue were significantly higher in I/R group than which in Sh group(P
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Medical Postgraduates
Année:
2003
Type:
Article