Comparison of BRAF mutation detection in patients with papillary thyroid microcarcinoma by ARMS and direct sequencing / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 1514-1516,1522, 2014.
Article
de Zh
| WPRIM
| ID: wpr-600301
Bibliothèque responsable:
WPRO
ABSTRACT
Objective:To investigate the sensitivity and the specificity of scorpions amplification refractory mutation system ( ARMS) in comparing with that of direct DNA sequencing in the detection of BRAF gene mutations in patients with papillary thyroid microcarcinoma.Methods:Direct sequencing and ARMS were used simultaneously to detect BRAF mutation status in 56 patients with PTMC.Results:BRAF mutations were identified in 46 cases with a mutation rate of 82.9%by ARMS,while in 18 cases with a mutation rate of 32.1%by direct sequencing.Besides,the sensitivity of ARMS was 100%and that of direct sequencing was 39.1%.There were significant differences of both mutation rate and sensitivity between two methods ( P<0.01 ).Conclusion: Compared to direct sequencing,ARMS gains a higher sensitivity in the detection of BRAF mutations in samples with tiny lesions.
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
langue:
Zh
Texte intégral:
Chinese Journal of Immunology
Année:
2014
Type:
Article