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Effect of advanced glycosylation end products on bFGF expression of cultured rabbit Müller cells / 中国免疫学杂志
Chinese Journal of Immunology ; (12): 1621-1623,1632, 2015.
Article de Zh | WPRIM | ID: wpr-603211
Bibliothèque responsable: WPRO
ABSTRACT
Objective:To observe the effects of different doses of advanced glycosylation end products ( AGEs ) on bFGF expression of cultured rabbit M üller cells in vitro.Methods:Immunocytochemistry and transmission electron microscopy methods were used identified cultured M üller cell.Immunocytochemistry method was used to semi-quantitate bFGF expression of retinal Müller cells at 640 μl/2 000 μl AGEs conditions.We observed effects of AGEs and PKC inhibitor Calphstion C on bFGF mRNA expression .Results:640 μl/2 000 μl AGEs stimulate bFGF expression of retinal Müller cell.Calphostin C inhibits bFGF mRNA increase stimulated by AGEs,and inhibition achieves strongest at concentration 50 nmol/L.Conclusion:AGEs can stimulate bFGF expression of Müller cell to exert the role of angiogenesis .bFGF mRNA expression may be regulated by activation of PKC pathway .
Mots clés
Texte intégral: 1 Indice: WPRIM Type d'étude: Prognostic_studies langue: Zh Texte intégral: Chinese Journal of Immunology Année: 2015 Type: Article
Texte intégral: 1 Indice: WPRIM Type d'étude: Prognostic_studies langue: Zh Texte intégral: Chinese Journal of Immunology Année: 2015 Type: Article