Detection and identification of a new bat bocavirus species in Yunnan / 军事医学
Military Medical Sciences
; (12): 207-212,233, 2016.
Article
Dans Zh
| WPRIM
| ID: wpr-603809
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective To discover more novel bat viruses and molecularly characterize bat-borne bocavirus diversity in Yunnan.Methods Twenty-six Aselliscus stoliczkanus were sampled in Jinghong , Yunnan, and subjected to viral metagenomic analysis.Specific PCR was used to detect any bocavirus in these samples based on the metagenomic result , while full genome was amplified and compared with other bocaviruses .Results and Conclusion Totally, 3 of the 26 (11.5%) bats were positive for bocavirus, the full genome of which contained 5203 nucleotides and could encode NS1, NP and VP1/VP2 proteins.Phylogenetic analysis showed that this virus shared up to 58.7% and 53.3% amino acid identities with canine bocavirus 1 and canine minute virus .According to ICTV criteria (85%amino acid sequence identity ) on a new species of bocavirus , this virus could be a novel species within genus Bocaparvovirus .This study provides important data to better understand viral diversity in bats and to uncover the relationship between bocavirus and its hosts .
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
langue:
Zh
Texte intégral:
Military Medical Sciences
Année:
2016
Type:
Article