Effect of miRNA-143 on homocysteine induced vascular smooth muscle cell proliferation / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 1097-1100,1101, 2016.
Article
Dans Zh
| WPRIM
| ID: wpr-604483
Responsable en Bibliothèque :
WPRO
ABSTRACT
Aim To explore the effect of miRNA-143 ( miR-1 4 3 ) on homocysteine ( Hcy ) induced-vascular smooth muscle cells ( VSMCs ) proliferation and the mechanism .Methods VSMCs were cultured and in-cubated with Hcy by using primary cultured method . Then, cells were treated with different concentrations of Hcy and folate .VSMCs proliferation was determined with MTT assay , miR-143 was measured by qRT-PCR, and methylation of miR-143 was determined with meth-ylated PCR.Results After cells were treated with dif-ferent concentrations of Hcy , the proliferation of VSMCs was significantly increased , mRNA expression of miR-143 was decreased and methylation of miR-143 was increased .The proliferation of VSMCs was signifi-cantly decreased when transfected VSMCs with miR-143 precursor , and cell proliferation was increased by using miR-143 inhibitor transfection .Conclusion Hy-pomethylation of miR-143 may inhibit VSMCs prolifera-tion.
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WPRIM
langue:
Zh
Texte intégral:
Chinese Pharmacological Bulletin
Année:
2016
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Article