Content Determination of Verbascoside in Naosaitong Honeyed Pill by HPLC / 中国药房
China Pharmacy
; (12): 2993-2994,2995, 2016.
Article
Dans Zh
| WPRIM
| ID: wpr-605740
Responsable en Bibliothèque :
WPRO
ABSTRACT
OBJECTIVE:
To establish a method for the content determination of verbascoside in Naosaitong honeyed pill.METHODS:
HPLC was performed on the column of Zorbax ODX with mobile phase of acetonitrile-0.1% phosphoric acid(16∶84, V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 334 nm,column temperature was 30 ℃,and the injection volume was 10 μl.RESULTS:
The linear range of verbascoside was 0.200-1.000 μg(r=0.999 8);RSDs of precision,stability and repro-ducibility tests were lower than 1%;recovery was 95.16%-99.78%(RSD=1.80,n=6).CONCLUSIONS:
The method is simple and accurate,and suitable for the content determination of verbascoside in Naosaitong honeyed pill.
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WPRIM
langue:
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Texte intégral:
China Pharmacy
Année:
2016
Type:
Article