Effects of ShenshuaiⅡ Prescription on Renal Morphology and Expression of TNF-α in Rats with 5/6 Kidney Ablation/Infarction / 中国中医药信息杂志

ABSTRACT

Objective To observe the effects ofShenshuaiⅡ Prescription on expressions of pathologic morphology and TNF-α in nephridial tissue of chronic renal failure (CRF) rats induced by 5/6th kidney (ablation/infarction, A/I); To investigate its possible mechanism of action.Methods 5/6 (A/I) nephrectomized CRF rat models were prepared and randomly divided into sham-operation group, model group, Western medicine group and TCM group. Each medication group was given relevant medicine. The levels of serum creatinine (SCr), blood urea nitrogen (BUN), hemoglobin (Hb) and endogenous creatinine clearance (Ccr) were measured after 60 days of administration. The histopathology of residual kidney was observed, and the expression of TNF-α in residual renal tissue was detected by immunohistochemistry.Results Compared with the model group, the levels of SCr and BUN significantly decreased, while Hb and Ccr significantly increased in each medication group (P<0.05,P<0.01). The pathological changes of the residual renal tissue were improved in each medication group. The expression of TNF-α protein in the rats of medication group was significantly lower than that in the Western medicine group (P<0.05, P<0.01).Conclusions henshuaiⅡ Prescription can improve renal function and alleviate changes of renal pathology in CRF rats. The mechanism of anti-renal interstitial fibrosis may be related to the improvement of renal ischemia and hypoxia and the regulation of the expression of TNF-α.