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Effect of FABP4 DNA methylation on abnormal lipid metabolism in trophocyte treated with L-NAME / 实用医学杂志
The Journal of Practical Medicine ; (24): 2255-2258, 2017.
Article de Zh | WPRIM | ID: wpr-617054
Bibliothèque responsable: WPRO
ABSTRACT
Objective To investigate the effect of fatty acid binding protein 4(FABP4)DNA methylation on abnormal lipid metabolism in placental trophoblastic dyslipidemia. Methods Human placental trophoblast cell line(HTR-8)was treated with L-NAME of 100 μmol/L for 48 h. The lipid content in placental trophoblasts was detected by chemical enzyme-colorimetry. The FABP4 DNA methylation level in placenta trophoblasts was detected by nested-touch down methylation specific PCR (NT-MSP). the mRNA and protein expression of DNMT1 and FABP4 were detected by qRT-PCR and Western Blot,respectively,in trophoblast cells. Results The lipid content in trophoblasts significantly increased as compared with the control(P < 0.05). Expression of FABP4 mRNA and protein increased(P < 0.05),while FABP4 methylation level and expression of DNMT1 significantly decreased (P<0.05)after treatment with L-NAME. Conclusions FABP4 DNA methylation is involved in the regulation of lipid metabolism in placental trophoblastic cells of hypertensive disorder complicating pregnancy.
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Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: The Journal of Practical Medicine Année: 2017 Type: Article
Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: The Journal of Practical Medicine Année: 2017 Type: Article