Reversion mutation in dark variants of luminous bacteria and its application in gene toxicant monitoring / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 180-2, 2002.
Article
de En
| WPRIM
| ID: wpr-634085
Bibliothèque responsable:
WPRO
ABSTRACT
The luminous intensity of dark variant (S1) separated from photobacterium phosphoreum (A2) was 1/10,000 less than that of wild-type. Ethidium bromide (EB) (0.6 mg/L), Mytomycin C (MC, 0.05 mg/L), 2-amino fluorene (2-AF, 1.0 mg/L) all could strongly induce reversion mutation for S1 within 24 h and increase reversion ratio significantly. The results of experiments indicated that these revertants had stable genetic characteristic and the mutation may take place at gene levels. The mutagenesis to S1 caused by EB, MC and 2-AF was detected and it may be used as a new rapid, simple and sensitive method for gene toxicant monitoring.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Photobacterium
/
Toxicologie
/
Transcription génétique
/
Variation génétique
/
Éthidium
/
Luciferases
/
Mesures de luminescence
/
Mitomycines
/
Mutagènes
/
Mutation
langue:
En
Texte intégral:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Année:
2002
Type:
Article