Standard and quantitative analysis of cyclin E threshold by cyclin E/DNA multiparameter flow cytometry / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 282-4, 2005.
Article
de En
| WPRIM
| ID: wpr-634268
Bibliothèque responsable:
WPRO
ABSTRACT
The threshold of cyclin E expression at G1/S boundary is a characteristic feature of cell cycle progressing. In this study, we tried to develop a quantitative approach to analyze cyclin E threshold by multiparameter flow cytometry. The expression of cyclin E in exponentially growing MOLT-4 cells was detected under different photomultiplier tube (PMT) voltages by cyclin E/DNA multiparameter flow cytometry. Additionally, cyclin E was detected in cells which were treated with caffeine and cycloheximide (CHX) under the same PMT voltage. Moreover, the expression of cyclin E in MOLT-4 cells was compared with that in JURKAT cells. Cyclin E threshold was quantified by formula B2/AxC (A, B, C indicates the minimum, threshold, and maximum of cyclin E fluorescence intensity, respectively). Results showed that in MOLT-4 cells, cyclin E threshold calculated by formula B2/AxC was invariable under different PMT settings. It was decreased in cells treated with caffeine and remained changeless in cells treated with cycloheximide. Cyclin E threshold in JURKAT cells was much lower than that in MOLT-4 cells. It was suggested that Formula B2/AxC we firstly set up could be used to analyze cyclin E expression threshold quantitatively.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Caféine
/
ADN tumoral
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Leucémie lymphoïde
/
Cycle cellulaire
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Cellules Jurkat
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Cycline E
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Cycloheximide
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Lignée cellulaire tumorale
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Cytométrie en flux
langue:
En
Texte intégral:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Année:
2005
Type:
Article