Development of porcine respiratory and reproductive syndrome virus replicon vector for foot-and-mouth disease vaccine
Clinical and Experimental Vaccine Research
; : 100-109, 2014.
Article
de En
| WPRIM
| ID: wpr-63816
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: Foot-and-mouth disease (FMD) is an economically important global animal disease. To control FMD virus (FMDV) outbreaks, a lot of different novel approaches have been attempted. In this study, we proposed a novel porcine reproductive and respiratory syndrome virus (PRRSV) as a replicon vector to express FMDV structural protein. MATERIALS AND METHODS: PRRSV infectious clone (PRRSVK418DM) was used to develop an expression vector through the reverse genetic manipulation of PRRSV; FMDVP12A3C gene of serotype O was synthesized and used for an antigen. MARC-145 cells (African green monkey kidney epithelial cell line) were used for electroporation mediated transfection. The transfection or the expression of P12A3C and N protein of PRRSV was analyzed by either replicon containing PRRSV alone or by co-infection of helper PRRSV. RESULTS: We constructed PRRSVK418DM replicon vector containing FMDVP12A3C, and genome sequences were confirmed by subsequent sequence analysis. In vitro expression of P12A3C and PRRSV N protein was confirmed by immunofluorescence antibody assay using antibodies specific for PRRSV N protein (anti-PRRSV N MAb), FMDV-VP1 (anti-VP1 MAb). CONCLUSION: The results indicate that PRRSV replicon vector can be a promising novel vector system to control FMDV and useful for vaccine development in the future.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Réplicon
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Virus
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Transfection
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Chlorocebus aethiops
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Épidémies de maladies
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Technique d'immunofluorescence
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Génome
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Clones cellulaires
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Analyse de séquence
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Électroporation
Limites du sujet:
Animals
langue:
En
Texte intégral:
Clinical and Experimental Vaccine Research
Année:
2014
Type:
Article