Application of Serum Protein Markers in Hirschsprung′s Disease / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)2004.
Article
de Zh
| WPRIM
| ID: wpr-639986
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To check serum protein of children′s Hirschsprung′s disease(HD) and sift specific protein marker which was used in constructing of HD screening and early diagnosis of serum protein fingerprint model.Methods Surface-enhanced laser desorption/ionization time of flight mass spectrometry(SELDI-TOF-MS) was applied to detect protein mass spectrometry of serum specimens in 82 cases(HD group 42 cases,20 cases of other types of obstruction,healthy control group 20 cases) and data were analyzed by bioinformatics methods(support vector machine).Results 1.For HD group and healthy control group:selected 3 M/Z in 3 221.7,5 639.2,6 884.2 protein markers were selected,HD early screening and diagnostic model was established,3 markers in HD low expression,the expressions of them in HD group and healthy control group were 378.29?273.34,295.65?159.38,444.13?254.06 and 1 428.18?1 192.61,1 039.60?785.64,1 115.72?680.48,respectively.There were significant differences in two groups(Pa0.05).Conclusions The establishment of serum protein fingerprint model of HD by SELDI-TOF-MS support vector machine could screen and diagnose HD early,which is a new method of better specificity,high sensitivity and is worthy of further research and application.
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Screening_studies
langue:
Zh
Texte intégral:
Journal of Applied Clinical Pediatrics
Année:
2004
Type:
Article