In vitro selection of DNA aptamers to Bacillus anthracis spores by SELEX / 军事医学科学院院刊
Bulletin of The Academy of Military Medical Sciences
; (6): 111-114, 2001.
Article
de Zh
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| ID: wpr-642451
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WPRO
ABSTRACT
Objective:To obtain oligonucleotide aptamers which can specifically bind to Bacillus anthracis spores by in vitro selection protocol-SELEX (system evolution of ligands by exponential enrichment).Methods:An in vitro synthesized 78 mer random DNA library (≤1014-15types of different DNAs ) was subjected to 15 rounds of selection using SELEX method against spores of B.anthracis vaccine strain A.16R. Binding of the aptamers to spores was visualized by biotin-streptavidin-horseradish peroxidase system.Results:PCR amplification band pattern of the first round selection was different from that of the ninth round. The binding assay demonstrated that D absorbance at 450 nm of the fifteenth round pool increased 9 times as compared with that of the first round , and the D absorbance increased with the increment of aptamers′ quantity binding to spores. Conclusions: A set of aptamers with considerable binding affinity to B.anthracis spores were successfully selected from the initial random DNA pool.
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Bulletin of The Academy of Military Medical Sciences
Année:
2001
Type:
Article