Scanning electronic microscope observation of bone marrow stem cells transfected by IGF-1 gene and cultured onto ostrich true bone ceramic / 实用口腔医学杂志
Journal of Practical Stomatology
; (6)2000.
Article
Dans Zh
| WPRIM
| ID: wpr-670870
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective:
To evaluate the biocompatibility of insulin-like growth factor I (IGF-1) gene transfected bone marrow stem cells (MSCs) with ostrich true bone ceramic (OTBC).Methods:
Rat MSCs were transfected with IGF-1 gene, and positive clones were selected by G418. The expression of IGF-1 protein in the MSCs was detected by immunocytochemical technique. The IGF-1 transfected MSCs were cultured with OTBC and the morphology of the cells was observed by scanning electronic microscope(SEM) at different time point.Results:
Immunohistochemical staining suggested that the IGF-1 protein was expressed in the IGF-1 transfected MSCs. The cells adhered to OTBC and stretched well after 24 h of culture. The IGF-1 transfected MSCs proliferated on the surface of OTBC with culture time.Conclusion:
The OTBC has a good biocompatibility with IGF-1 transfected MSCs.
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Practical Stomatology
Année:
2000
Type:
Article