Effects of estrogen on sarcoplasmic reticulum Ca~(2+)-ATPase activity and gene expression in genioglossal muscle of female rats / 实用口腔医学杂志

ABSTRACT

Objective:To investigate the effects of estrogen on sarcoplasmic reticulum (SR) Ca2+-ATPase activity and gene expression in female rats genioglossal muscle (GG), and explore the possible molecular biology mechanism.Methods:30 male rats were randomly divided into 3 groups:Control group, ovariectomy(OVX)group, ovariectomized animals receiving estradiol replacement (OVX+E2) group. The rats were sacrificed 6 weeks after surgery, and the genioglossus was taken out for analysis. Ca2+-ATPase activity was measured by detecting inorganic phosphorus ion, and SR Ca2+-ATPase (SERCA) mRNA level was observed using fluorescence quantitative reverse transcription-polymerase chain reaction (FQ RT-PCR) method. Results:Compared to the control group, SR Ca2+-ATPase activity of OVX group remarkably reduced(P0.05).Conclusion:Serum level of estrogen of adult female rats may influence the function of genioglossal muscle by influencing SR Ca2+-ATPase activity and the SERCA mRNA expression.