Study on phenotypic screening and molecular genetics of rare blood type Jk(a-b-) in donors of Fujian province / 临床检验杂志
Chinese Journal of Clinical Laboratory Science
; (12): 173-177, 2018.
Article
Dans Zh
| WPRIM
| ID: wpr-694821
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective To investigate the frequency of Jk(a-b-) phenotype of Kidd blood type system in blood donors of Fujian province and its genetic characteristics.Methods The Jk (a-b-) phenotype in the blood samples obtained from 180 626 donors were screened by using urea lysis assay and the suspected Jk(a-b-) pbenotype individuals were confirmed using conventional serological method.The genomic DNA covering the sequence from exon 1 to exon 11 of JK gene and respective flanking area(50-150 bp),as well as the promoter were amplified by polymerase chain reaction,and the products of PCR were directly sequenced.The genotypes of 7 SNPs of JK gene in the blood samples from 200 blood donors of Fujian province were detected by SNaPshot assay.Results Of 180 626 blood donors,15 cases with Jk(a-b-) phenotype were identified.The genomic analysis for the 15 cases revealed the four recessive JK-null alleles,i.e.,JK*B(IVS5-1G>A),JK*B(896G>A),JK*A(130G>A,220A >G) and JK* B(130G >A,956C > T) were observed with frequency of 66.67%,23.33%,6.67% and 3.33%,respectively.SNaPshot results showed the frequency of JK * B (IVS5-1 G > A) was 0.75 % and G130A was the common polymorphism.No A220G,C222T,C956T and G896A mutation was found in the 200 blood donors.Conclusion The frequency of Jk (a-b-) blood type in the donors of Fujian population was estimated about 0.008%.JK * B(IVS5-1G > A) and JK * B(896G > A) alleles may be the predominate circulating genes in Fujian population with Jk (a-b-) phenotype.Direct DNA sequencing revealed a novel allele leading to JK-null,SLC14A1 130A,220G.
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
langue:
Zh
Texte intégral:
Chinese Journal of Clinical Laboratory Science
Année:
2018
Type:
Article