Analysis of aztreonam and metronidazole in blood by ultra performance liquid chromatography-tandem mass spectrometry / 复旦学报（医学版）

ABSTRACT

Objective To establish a UPLC-MS/MS method for the determination of aztreonam and metronidazole in blood.Methods Cefradine and chloramphenicol were used as the internal standard.Blood sample was made by protein precipitation with acetonitrile,and then was seprated by HPLC.Multiple reaction monitoring mode (MRM) for the parent ion and ion monitoring was used for each compound.Results The linearity of aztreonam in the range from 10 to 5 000 ng/mL and metronidazole in the range from 1 to 1 000 ng/mL were good (r＞0.995).The quantification limit of the method for aztreonam and metronidazole were 5 and 0.5 ng/mL,respectively (S/N =3 ∶ 1).The inter-day and intra-day relative standard deviations (RSD) were less than 10 ％.The established method was used to detect aztreonam and metronidazole in a medical dispute case,the concentrations of aztreonam and metronidazole in the blood were 438 ng/mL and 15 μg/mL,respectively.Conclusions The method developed in the study is simple,sensitive and reproducible with its successful application into the qualitative and quantitative analysis of aztreonam and metronidazole.