Association of interleukin-32 gene polymorphism and its serum levels with genetic susceptibility in patients with multiple sclerosis / 中国免疫学杂志

ABSTRACT

Objective:To investigate interleukin-32 (IL-32)(rs28372698A/T,rs12934561C/T and rs11861531C/T) genetic susceptibility in patients with multiple sclerosis(MS)by case-control study,which provides a theoretical foundation for high-risk population with MS.Methods:A total 580 MS patients and 650 healthy controls were included in this study,polymerase chain reaction-single base extension (PCR-SEB) was used to test DNA sequencing,and serum levels of IL-32 were determined by enzyme-linked im-munosorbent assay.Results:The genotype and allele frequency of IL-32 (rs28372698A/T) had significantly differences compared with healthy controls (P=0.007,P=0.033),however,there were no statistical differences in rs12934561C/T and rs11861531C/T between the two groups (P>0.05).T-T-T haploid genotype in patients with MS was higher than control groups(P=0.012),and T-T-T haploid genotype was associated with increased risk of MS(OR=1.968,95% CI:1.968-1.352).Serum levels of IL-32 in patients with MS was increased compared with control groups[(399.08 ± 156.85)pg/ml vs (239.99 ± 88.35)pg/ml,P= 0.001].The serum IL-32 concentrations in MS patients with AT and TT genotype were higher compared with MS patient with AA genotype[(465.53±172.40) pg/ml vs(295.86±103.96)pg/ml,P<0.01;(491.15±133.65)pg/ml vs(295.86±103.96)pg/ml,P<0.01].Conclusion:Our study found that an association between IL-32(rs28372698) gene polymorphism and MS,and serum levels of IL-32 were influenced by IL-32 gene polymorphism in patients with MS,suggesting a theoretical basis for individualized diagnosis and treatment of MS patients.