Effect of XBP1/CHOP/Puma signaling pathway on apoptosis in cardiomyocyte of high glucose environment / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effect of X-box binding protein 1(XBP1) on the apoptosis of H9C2 cardiomyocytes under high glucose by XBP1/CHOP/Puma signaling pathway.Methods: The experiment were divided into four groups:low glucose group (LG group),high glucose group (HG group),high glucose+siRNA-NC group (HG+NC group),high glucose+siRNA-XBP1 (HG +siRNA).The expression of XBP1,C/EBP homologous protein (CHOP),p53 upregulated modulator of apoptosis(Puma), Caspase-3 in the cells after transfected were detected by Western blot;CCK-8 were used to detect the cell proliferation.Cell apoptosis was detected by flow cytometry.Results:siRNA-XBP1 reduced the expression of XBP1 in cardiomyocytes(P<0.05).Compared with LG group,the survival rate of HG group,HG +NC group and HG +siRNA group was significantly decreased (P<0.05),and the apoptosis rate was significantly increased(P<0.05).Compared with HG group,the survival rate of HG+siRNA group was significantly increased (P<0.05),and the apoptosis rate was significantly decreased(P<0.05).XBP1,CHOP,Puma in HG group,HG +NC group and HG+siRNA group was significantly higher than in LG group(P<0.05).Compared with HG group,the expression of XBP1,CHOP, Puma and Caspase-3 in HG+siRNA group was significantly lower(P<0.05).Conclusion:XBP1 inhibits cardiomyocyte apoptosis and promotes proliferation by regulating XBP1/CHOP/Puma signaling pathway.