Application of rhodamine-based fluorescent molecular probes in visualization of cellular pyruvic acid / 中国药科大学学报
Journal of China Pharmaceutical University
; (6): 79-86, 2018.
Article
de Zh
| WPRIM
| ID: wpr-704325
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ABSTRACT
In order to conduct the visualized research of cellular pyruvic acid(PA),several rhodamine-based probes were designed to react with PA by a Schiff-base reaction in aqueous media and exhibit strong fluorescence emission and a pink color owing to the ring-opening of the corresponding spirolactam.The results showed that R6G-NH2had a highest sensitive response to PA with a linearly relationship between the enhancement of fluores-cence intensity at 565 nm and the concentration of PA from 0.2 μmol/L to 120 μmol/L.According to the princi-ple of 3σ,a limit of detection was obtained as low as 0.1 μmol/L.Furthermore,the response of R6G-NH2to PA was hardly affected by conventional biomolecules and ionic species.Therefore,R6G-NH2was successfully used in fluorescence imaging of exogenous PA in non-small cell lung cancer cell A549 and renal epithelial cell 293T,and endogenous PA induced by bisphenol A in epithelial cell 293T.
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WPRIM
langue:
Zh
Texte intégral:
Journal of China Pharmaceutical University
Année:
2018
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Article