Application of rhodamine-based fluorescent molecular probes in visualization of cellular pyruvic acid / 中国药科大学学报

ABSTRACT

In order to conduct the visualized research of cellular pyruvic acid(PA),several rhodamine-based probes were designed to react with PA by a Schiff-base reaction in aqueous media and exhibit strong fluorescence emission and a pink color owing to the ring-opening of the corresponding spirolactam.The results showed that R6G-NH2had a highest sensitive response to PA with a linearly relationship between the enhancement of fluores-cence intensity at 565 nm and the concentration of PA from 0.2 μmol/L to 120 μmol/L.According to the princi-ple of 3σ,a limit of detection was obtained as low as 0.1 μmol/L.Furthermore,the response of R6G-NH2to PA was hardly affected by conventional biomolecules and ionic species.Therefore,R6G-NH2was successfully used in fluorescence imaging of exogenous PA in non-small cell lung cancer cell A549 and renal epithelial cell 293T,and endogenous PA induced by bisphenol A in epithelial cell 293T.