Your browser doesn't support javascript.
loading
Cutaneous Microflora from Geographically Isolated Groups of Bradysia agrestis, an Insect Vector of Diverse Plant Pathogens
Mycobiology ; : 160-171, 2017.
Article de En | WPRIM | ID: wpr-729299
Bibliothèque responsable: WPRO
ABSTRACT
Larvae of Bradysia agrestis, an insect vector that transports plant pathogens, were sampled from geographically isolated regions in Korea to identify their cutaneous fungal and bacterial flora. Sampled areas were chosen within the distribution range of B. agrestis; each site was more than 91 km apart to ensure geographical segregation. We isolated 76 microbial (fungi and bacteria) strains (site 1, 29; site 2, 29; site 3, 18 strains) that were identified on the basis of morphological differences. Species identification was molecularly confirmed by determination of universal fungal internal transcribed spacer and bacterial 16S rRNA gene sequences in comparison to sequences in the EzTaxon database and the NCBI GenBank database, and their phylogenetic relationships were determined. The fungal isolates belonged to 2 phyla, 5 classes, and 7 genera; bacterial species belonged to 23 genera and 32 species. Microbial diversity differed significantly among the geographical groups with respect to Margalef's richness (3.9, 3.6, and 4.5), Menhinick's index (2.65, 2.46, and 3.30), Simpson's index (0.06, 0.12, and 0.01), and Shannon's index (2.50, 2.17, and 2.58). Although the microbial genera distribution or diversity values clearly varied among geographical groups, common genera were identified in all groups, including the fungal genus Cladosporium, and the bacterial genera Bacillus and Rhodococcus. According to classic principles of co-evolutionary relationship, these genera might have a closer association with their host insect vector B. agrestis than other genera identified. Some cutaneous bacterial genera (e.g., Pseudomonas) displaying weak interdependency with insect vectors may be hazardous to agricultural environments via mechanical transmission via B. agrestis. This study provides comprehensive information regarding the cutaneous microflora of B. agrestis, which can help in the control of such pests for crop management.
Sujet(s)
Mots clés
Texte intégral: 1 Indice: WPRIM Sujet Principal: Plantes / Bacillus / Rhodococcus / Cladosporium / Gènes d'ARN ribosomique / Bases de données d'acides nucléiques / Biodiversité / Vecteurs insectes / Insectes / Corée Pays comme sujet: Asia langue: En Texte intégral: Mycobiology Année: 2017 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Plantes / Bacillus / Rhodococcus / Cladosporium / Gènes d'ARN ribosomique / Bases de données d'acides nucléiques / Biodiversité / Vecteurs insectes / Insectes / Corée Pays comme sujet: Asia langue: En Texte intégral: Mycobiology Année: 2017 Type: Article