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Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
Mycobiology ; : 258-262, 2012.
Article de En | WPRIM | ID: wpr-729695
Bibliothèque responsable: WPRO
ABSTRACT
cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 microM within 90 min.
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Texte intégral: 1 Indice: WPRIM Sujet Principal: Composés organométalliques / Oxidoreductases / Peroxidases / Pichia / Magenta I / ADN complémentaire / Myeloperoxidase / Phanerochaete / Alcohol oxidoreductases / Glyoxal langue: En Texte intégral: Mycobiology Année: 2012 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Composés organométalliques / Oxidoreductases / Peroxidases / Pichia / Magenta I / ADN complémentaire / Myeloperoxidase / Phanerochaete / Alcohol oxidoreductases / Glyoxal langue: En Texte intégral: Mycobiology Année: 2012 Type: Article