VWF-A1A2A3-mediated expression of P-selectin in platelets under flow shear stress / 医用生物力学
Journal of Medical Biomechanics
; (6): 188-193,204, 2017.
Article
Dans Zh
| WPRIM
| ID: wpr-735855
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective To investigate the effect of fluid shear stress (FSS) on VWF-A1A2A3-mediated expression of platelet surface P-selectin.Methods Using a parallel plate flow chamber system and mouse anti-human CD62PFITC as the indicator of P-selectin expression,the alteration of platelet P-selectin expression level with increasing exposure time under different FSS conditions (0,1,2 Pa) specifically mediated by VWF-A1A2A3 was observed and analyzed by fluorescence microscope to obtain the activation characteristics.Results FSS triggered platelet activation and P-selectin expression.The activation ratio of platelet was positively regulated by FSS and their exposure time,reaching the maximum value,9.42% and 14.59% under FSS of 1 Pa and 2 Pa,respectively.The level of P-selectin expression exhibited two-phase tendency with the shear stress-exposure time increasing,uplifted at first,then decreased,with the best action time at 7.5 min.The fluorescence peak intensity increased when FSS was enhanced.Conclusions The level of platelet P-selectin expression is co-regulated by VWF-A1A2A3 and FSS,and is closely related to force-signaling exposure time.
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WPRIM
langue:
Zh
Texte intégral:
Journal of Medical Biomechanics
Année:
2017
Type:
Article