Genetic characterization of norovirus isolated in an outbreak of gastroenteritis in Jiangsu province / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 72-74, 2018.
Article
de Zh
| WPRIM
| ID: wpr-737920
Bibliothèque responsable:
WPRO
ABSTRACT
Objective: To analyze the genetic characterization of norovirus isolated in an outbreak of gastroenteritis in Jiangsu province. Methods: Extracted viral RNA from the swab samples of cases of acute gastroenteritis outbreak in Jiangsu province on December 16-27, 2016 was reversely transcribed to cDNA, and partial RNA-dependent RNA polymerase sequence and complete capsid sequence (VP1) were amplified by RT-PCR. Amplification products were sequenced for the analysis of genetic characteristics. Results: Based on sequence alignment, the variant shared a high level of identity with the strain GⅡ.g isolated in Spain and Finland (98.7%) in the RNA-dependent RNA polymerase region, and with the strain GⅡ.1 isolated in American (99.4%) in the VP1. The recombination was determined by using software Simplot, and the breakpoint of recombination was located in the ORF1/2 overlap region at position 5 106 of VP1. The result of amino acids alignment in capsid region showed that there were no mutations in the amino acids of the predicted epitopes and receptor binding site Ⅰ-Ⅲ, but a unique amino acid change was detected at position 132 (N-S). Conclusion: The norovirus isolated in the outbreak of gastroenteritis in Jiangsu province was a rare recombinant norovirus variant GⅡ.g-GⅡ.1.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phylogenèse
/
ARN viral
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Épidémies de maladies
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Analyse de séquence d'ADN
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Infections à Caliciviridae
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RT-PCR
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Norovirus
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Protéines de capside
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Gastroentérite
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Génotype
Type d'étude:
Prognostic_studies
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Epidemiology
Année:
2018
Type:
Article