Regulation of Matrix Metalloproteinase 2 Expression by an Adenosine A1 Agonist in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
; : 946-952, 2018.
Article
Dans Ko
| WPRIM
| ID: wpr-738482
Responsable en Bibliothèque :
WPRO
ABSTRACT
PURPOSE:
We investigated the extent of adenosine A1 agonist-induced expression and regulation of matrix metalloproteinase 2 (MMP-2) synthesis in human trabecular meshwork cells (HTMC).METHODS:
Primary HTMC cultures were exposed to 0.1 or 1.0 µM N6-cyclohexyladenosine (CHA) for 2 h in the presence or absence of an inhibitor thereof, 8-cyclopentyl-1,3-dimethylxanthine (CPT). The expression level of mRNA encoding MMP-2 was assessed via reverse transcription-polymerase chain reaction, and the levels of tissue inhibitor of metalloproteinase 2 (TIMP2) and membrane-type-1 MMP (MT1-MMP) measured by Western blotting. The permeability of the HTMC monolayer was assessed with the aid of carboxyfluorescein.RESULTS:
CHA at 1.0 µM increased the permeability of the HTMC monolayer (p = 0.003) and CHA at both 0.1 and 1.0 µM significantly increased MMP-2 mRNA expression, which was inhibited by co-exposure to CPT (all p < 0.05). CHA increased MMP-2 activity, decreased that of TIMP2, and increased that of MT1-MMP (all p < 0.05).CONCLUSIONS:
CHA increased the permeability of the HTMC monolayer and increased MMP-2 activity, decreased TIMP2 activity, and increased MT1-MMP activity. Thus, regulation of TIMP2 and MT1-MMP expression may be involved in the adenosine A1 agonist-induced increase in MMP-2 activity.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Perméabilité
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Réseau trabéculaire de la sclère
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ARN messager
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Adénosine
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Technique de Western
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Inhibiteur tissulaire de métalloprotéinase-2
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Matrix metalloproteinase 2
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Matrix metalloproteinase 14
Limites du sujet:
Humans
langue:
Ko
Texte intégral:
Journal of the Korean Ophthalmological Society
Année:
2018
Type:
Article