The inhibitory effect of FA-MNP-MMP-9-ASODN nanocomposite on nasopharyngeal carcinoma in vitro / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 607-611, 2014.
Article
de Zh
| WPRIM
| ID: wpr-748168
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE@#To observe the inhibitory effect of FA-MNP-MMP-9-ASODN nanocomposite on nasopharyngeal carcinoma (NPC) HNE-1 cell in vitro.@*METHOD@#To observe the MMP-9 mRNA and protein expression levels, proliferation ability, cell apoptosis and invasion ability of HNE-1 cell 48 hours after FA-MNP-MMP-9-ASODN transfection by RT-PCR, Western-blot, MTT assay, flow cytometry and Matrigel invasion test.@*RESULT@#MMP-9 mRNA and protein expression in HNE-1 cell of FA-MNP-MMP-9-ASODN group was significantly decreased compared to control group and nonsense sequence group of FA-MNP-MMP-9-NSODN. At the same time, for the HNE-1 cell in FA-MNP-MMP-9-ASODN group, growth inhibition rate was about 35.66%, proliferation activity significantly decreased compared to the control group and the nonsense sequence group, cell cycle was also inhibited, cell apoptosis rate was about 12.60%, the apoptosis rate was significantly higher than that in the control group and the nonsense sequence group. Invasion assay showed that the transmembrane cells in FA-MNP-MMP-9-ASODN group were about 21.00, significantly lower than that in the control group and the nonsense sequence group.@*CONCLUSION@#By inhibiting the expression of MMP-9, FA-MNP-MMP-9-ASODN nanocomposites could reduce NPC cell proliferation and invasion ability, and promote apoptosis, it had a good inhibitory effect in vitro.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
/
Carcinomes
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Transfection
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Tumeurs du rhinopharynx
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Apoptose
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Matrix metalloproteinase 9
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Lignée cellulaire tumorale
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Prolifération cellulaire
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Nanocomposites
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Cancer du nasopharynx
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Année:
2014
Type:
Article