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Comparison of ELISA and pharmacia UNICAP detection reagent for house dust mite specific IgG4 / 临床耳鼻咽喉头颈外科杂志
Article de Zh | WPRIM | ID: wpr-749263
Bibliothèque responsable: WPRO
ABSTRACT
OBJECTIVE@#To compare the ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 in clinic.@*METHOD@#The stability of ELISA reagent was studied first with parameters of intra and inter assay variations (%CV). Calibration curve of the two reagents was compared. Sixty-five serum samples before or after standardized specific immunotherapy were examined with the two methods, correlation coefficient was calculated.@*RESULT@#The stability of ELISA reagent was good with low intra and inter assay variations (3.48% and 5.78%). Calibration curve of the two reagents was similar. The correlation coefficient was 0.93 between the two test methods.@*CONCLUSION@#The results of ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 were highly consistent. The ELISA reagents could be applied in clinic.
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Sang / Calibrage / Immunoglobuline G / Test ELISA / Pyroglyphidae / Allergie et immunologie / Indicateurs et réactifs / Méthodes Type d'étude: Diagnostic_studies Limites du sujet: Animals / Humans langue: Zh Texte intégral: Lin chuang er bi yan hou ke za zhi Année: 2013 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Sang / Calibrage / Immunoglobuline G / Test ELISA / Pyroglyphidae / Allergie et immunologie / Indicateurs et réactifs / Méthodes Type d'étude: Diagnostic_studies Limites du sujet: Animals / Humans langue: Zh Texte intégral: Lin chuang er bi yan hou ke za zhi Année: 2013 Type: Article