Epidural Fat-Derived Mesenchymal Stem Cell: First Report of Epidural Fat-Derived Mesenchymal Stem Cell
Asian Spine Journal
; : 361-367, 2019.
Article
Dans En
| WPRIM
| ID: wpr-762957
Responsable en Bibliothèque :
WPRO
ABSTRACT
STUDY DESIGN:
Experimental study.PURPOSE:
To determine whether epidural fat (EF) tissue contains mesenchymal stem cells (MSC). OVERVIEW OF LITERATURE Spine surgeons are unaware of the contents of EF tissue and the reason for its presence between the ligamentum flavum and the dura mater; therefore, EF tissues are routinely eliminated during surgical procedures. However, EF removal causes certain postoperative problems, such as post-laminectomy syndrome. We hypothesized that the EF tissue may play a significant supportive role for the neural structures and other nearby conditions.METHODS:
EF tissues were obtained from consenting patients (n=3) during posterior decompression surgery of the lumbar spine. The primary cells were isolated and cultured as per previously described methods with some modifications, and the cell morphology and cumulation were examined. Thereafter, reverse transcription–polymerase chain reaction (RT-PCR), a fluorescence-activated cell sorting (FACS) analysis, and differentiation potency for differentiation into osteoblasts, chondroblasts, and adipocytes were investigated to identify whether the cells derived from EF are MSC.RESULTS:
The cells from the EF tissue had a fibroblast or neuron-like morphology that persisted until the senescence at p18. MSC-specific genes, such as OCT4, SOX2, KLF4, MYC, and GAPDH were expressed in the RT-PCR study, while MSC-specific surface markers such as CD105, CD90, and CD73 were exhibited in the FACS analysis. The differentiation properties of EF-MSC for differentiation into the three types of cells (osteoblast, chondroblast, and adipocyte) were also confirmed.CONCLUSIONS:
Based on the cell culture, FACS analysis, RT-PCR analysis, and differentiation potent outcomes, all the features of the cells corresponded to MSC. This is the first study to identify EF-MSC derived from the EF tissue.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Ostéoblastes
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Rachis
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Vieillissement
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Ligament jaune
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Adipocytes
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Techniques de culture cellulaire
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Chondrocytes
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Décompression
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Dure-mère
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Cellules souches mésenchymateuses
Limites du sujet:
Humans
langue:
En
Texte intégral:
Asian Spine Journal
Année:
2019
Type:
Article