Magnoliae Cortex and maize modulate Porphyromonas gingivalis-induced inflammatory reactions
Journal of Periodontal & Implant Science
; : 70-83, 2018.
Article
Dans En
| WPRIM
| ID: wpr-766056
Responsable en Bibliothèque :
WPRO
ABSTRACT
PURPOSE:
The aim of this study was to evaluate the capacity of single and combined applications of the bark of the stems and roots of Magnolia officinalis Rehd. et Wils. (Magnoliae Cortex) and Zea mays L. (maize) to modulate inflammation in RAW 264.7 cells stimulated with Porphyromonas gingivalis.METHODS:
RAW 264.7 cells were stimulated with P. gingivalis, and Magnoliae Cortex and/or maize was added. Cytotoxicity and the capacity to modulate inflammation were determined with a methylthiazol tetrazolium (MTT) assay, nitrite production, enzyme-linked immunosorbent assay (ELISA), and western blotting.RESULTS:
Treatment with Magnoliae Cortex and/or maize inhibited nuclear transcription factor κB (NF-κB) pathway activation and nuclear p44/42 mitogen-activated protein kinase (MAPK) and inducible nitric oxide synthase (iNOS) protein expression in P. gingivalis-stimulated RAW 264.7 cells. Moreover, the treatments suppressed cytokines (prostaglandin E2 [PGE2], interleukin [IL]-1β, and IL-6) and nitrite production.CONCLUSIONS:
Both Magnoliae Cortex and maize exerted an anti-inflammatory effect on P. gingivalis-stimulated RAW 264.7 cells, and this effect was more pronounced when the extracts were combined. These findings show that these extracts may be beneficial for slowing the progression of periodontal disease.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Maladies parodontales
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Protein kinases
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Facteurs de transcription
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Test ELISA
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Technique de Western
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Cytokines
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Interleukines
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Porphyromonas
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Porphyromonas gingivalis
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Zea mays
langue:
En
Texte intégral:
Journal of Periodontal & Implant Science
Année:
2018
Type:
Article