Your browser doesn't support javascript.
loading
Transcriptional activation of glucose transporter 1 in orthodontic tooth movement-associated mechanical response / 国际口腔科学杂志·英文版
Article de En | WPRIM | ID: wpr-772289
Bibliothèque responsable: WPRO
ABSTRACT
The interplay between mechanoresponses and a broad range of fundamental biological processes, such as cell cycle progression, growth and differentiation, has been extensively investigated. However, metabolic regulation in mechanobiology remains largely unexplored. Here, we identified glucose transporter 1 (GLUT1)-the primary glucose transporter in various cells-as a novel mechanosensitive gene in orthodontic tooth movement (OTM). Using an in vivo rat OTM model, we demonstrated the specific induction of Glut1 proteins on the compressive side of a physically strained periodontal ligament. This transcriptional activation could be recapitulated in in vitro cultured human periodontal ligament cells (PDLCs), showing a time- and dose-dependent mechanoresponse. Importantly, application of GLUT1 specific inhibitor WZB117 greatly suppressed the efficiency of orthodontic tooth movement in a mouse OTM model, and this reduction was associated with a decline in osteoclastic activities. A mechanistic study suggested that GLUT1 inhibition affected the receptor activator for nuclear factor-κ B Ligand (RANKL)/osteoprotegerin (OPG) system by impairing compressive force-mediated RANKL upregulation. Consistently, pretreatment of PDLCs with WZB117 severely impeded the osteoclastic differentiation of co-cultured RAW264.7 cells. Further biochemical analysis indicated mutual regulation between GLUT1 and the MEK/ERK cascade to relay potential communication between glucose uptake and mechanical stress response. Together, these cross-species experiments revealed the transcriptional activation of GLUT1 as a novel and conserved linkage between metabolism and bone remodelling.
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Desmodonte / Pharmacologie / Mouvement dentaire / Phénomènes biomécaniques / Immunohistochimie / Activation de la transcription / Cellules cultivées / Technique de Western / Remodelage osseux / Rat Sprague-Dawley Type d'étude: Prognostic_studies Limites du sujet: Animals / Humans langue: En Texte intégral: International Journal of Oral Science Année: 2018 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Desmodonte / Pharmacologie / Mouvement dentaire / Phénomènes biomécaniques / Immunohistochimie / Activation de la transcription / Cellules cultivées / Technique de Western / Remodelage osseux / Rat Sprague-Dawley Type d'étude: Prognostic_studies Limites du sujet: Animals / Humans langue: En Texte intégral: International Journal of Oral Science Année: 2018 Type: Article