Streptococcus mutans activates the AIM2, NLRP3 and NLRC4 inflammasomes in human THP-1 macrophages / 国际口腔科学杂志·英文版
International Journal of Oral Science
; (4): 23-23, 2018.
Article
Dans En
| WPRIM
| ID: wpr-772292
Responsable en Bibliothèque :
WPRO
ABSTRACT
Streptococcus mutans (S. mutans), a major aetiologic agent of dental caries, is involved in systemic diseases, such as bacterial endocarditis, if it enters the bloodstream through temporary bacteraemia. Interleukin (IL)-1β, a proinflammatory cytokine, is related to the host defences against pathogens, and its synthesis, maturation, and secretion are tightly regulated by the activation of the inflammasome, an inflammatory signalling complex. This study examined the signalling mechanism of IL-1β secretion and the inflammasome pathway induced by S. mutans to explain the molecular mechanism through which systemic infection by oral streptococci can occur. After infection of THP-1 cells with S. mutans, the expression of inflammasome components was detected using various methods. S. mutans induced IL-1β secretion via caspase-1 activation, and S. mutans-induced IL-1β secretion required absent in melanoma (AIM2), NLR family pyrin domain-containing 3 (NLRP3) and NLR family CARD domain-containing 4 (NLRC4) inflammasome activation. In particular, the S. mutans-induced NLRP3 inflammasome was mediated by adenosine triphosphate (ATP) release, potassium depletion and lysosomal damage. Our study provides novel insight into the innate immune response against S. mutans infection.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Streptococcus mutans
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Protéines de liaison au calcium
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Test ELISA
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Transduction du signal
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Technique de Western
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Facteur de nécrose tumorale alpha
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Caspase-1
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Protéines de liaison à l'ADN
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Allergie et immunologie
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Interleukine-1 bêta
Limites du sujet:
Humans
langue:
En
Texte intégral:
International Journal of Oral Science
Année:
2018
Type:
Article