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Establishment of Flow Cytometric Immunobead Array for Detecting Plasma Von Willebrand Factor Activity and Its Clinical Application in the Prognosis of Ischemic Stroke / 中国实验血液学杂志
Article de Zh | WPRIM | ID: wpr-774334
Bibliothèque responsable: WPRO
ABSTRACT
OBJECTIVE@#To establish a novel flow cytometric immunobead array (FCIA) for detecting plasma von Willebrand factor activity (vWF:GPIbR) and apply it in ischemic stroke (IS).@*METHODS@#Microspheres coated with anti-human platelet glycoprotein Ibα (GPIbα) monoclonal antibody SZ151 IgG, were incubated with recombinant fragment of GPIbα, then added ristocetin and plasma, finally incubated with FITC-conjugated sheep-anti-human vWF IgG polyclonal antibody, and detected by flow cytometry. vWF antigen (vWF:Ag), vWF:GPIbR, and vWF collagen binding assay (vWF:CB) were also included for evaluating vWF levels in IS patients.@*RESULTS@#The intra-assay coefficient variations (CVs) and inter-assay CVs of FCIA were 7.7% and 13.5%, respectively. The slope of the linear regression was 0.9739 (r=0.855, P<0.001), and the Bland-Altman bias was 9.95%, indicating a good correlation between FCIA and ELISA. The FCIA had better sensitivity, specificity and accuracy as compared with those by ELISA (P<0.05). The levels of vWF:Ag, vWF:GPIbR and vWF:CB in IS patients were significantly higher in comparison with those in healthy controls (H=7.8, 6.4, 6.2, respectively, P<0.01), the level of vWF:GPIbR in IS patients positively correlated with levels of vWF:Ag, high-sensitivity C-reactive protein, Autar score and hospitalization time.@*CONCLUSION@#The FCIA for detecting plasma vWF:GPIbR is more specific and accurate than ELISA. The vWF:GPIbR is involved in the paroxysm of IS, which could be used to evaluate the risk of thrombosis in IS patients.
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Pronostic / Maladies de von Willebrand / Facteur de von Willebrand / Ovis / Encéphalopathie ischémique / Accident vasculaire cérébral / Cytométrie en flux Type d'étude: Prognostic_studies Limites du sujet: Animals / Humans langue: Zh Texte intégral: Journal of Experimental Hematology Année: 2019 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Pronostic / Maladies de von Willebrand / Facteur de von Willebrand / Ovis / Encéphalopathie ischémique / Accident vasculaire cérébral / Cytométrie en flux Type d'étude: Prognostic_studies Limites du sujet: Animals / Humans langue: Zh Texte intégral: Journal of Experimental Hematology Année: 2019 Type: Article