Fingerprinting and multi-indicator quantitative analysis of Mongolian drug Digeda-4 decoction / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 3962-3969, 2018.
Article
de Zh
| WPRIM
| ID: wpr-775391
Bibliothèque responsable:
WPRO
ABSTRACT
To establish the high performance liquid chromatography (HPLC) fingerprint for Digeda-4 decoction (DGD-4D), determine the contents of aesculetin, geniposide, picroside Ⅰ, picroside Ⅱ and ellagicacid in DGD-4D, and provide the scientific foundation for quality control of DGD-4D. The analysis was performed on Diamonsil(2) C₁₈ (4.6 mm×250 mm,5 μm) column, with methanol-0.1% phosphoric acid aqueous solution as mobile phase for gradient elution. The flow rate was 1.0 mL·min⁻¹; injection size was 10 μL; temperature was maintained at 30 °C, and the detection wavelength was set at 254 nm. The common mode of DGD-4D HPLC fingerprint was established, and the hidden information was analyzed by Chemometrics. Chromatographic peaks for DGD-4D were identified by HPLC and quantitative analysis was conducted for characteristic peaks. There were 17 common peaks in the fingerprints and the similarity of the fingerprints was over 0.9 in all 15 batches. The samples were broadly divided into four kinds by principal component analysis and clustering analysis. Four marker compounds were verified by partial least squares discriminant analysis, and No. 9, 12 and 14 peaks were identified as geniposide, picroside Ⅱ, and picroside Ⅰ respectively. The average recoveries were in the range of 95.91%-97.31%. The HPLC fingerprint method for content determination is reliable, accurate, rapid, simple, and reproducible, and can be used as one of the effective methods to control the quality of DGD-4D.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Contrôle de qualité
/
Normes de référence
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Médicaments issus de plantes chinoises
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Chromatographie en phase liquide à haute performance
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Cinnamates
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Analyse en composantes principales
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Iridoïdes
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Méthanol
/
Glucosides d'iridoïdes
Type d'étude:
Prognostic_studies
langue:
Zh
Texte intégral:
Zhongguo Zhong Yao Za Zhi
Année:
2018
Type:
Article