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Effects of shading on key enzyme genesexpression and accumulation of saponins in Panax japonicus var. major / 中国中药杂志
Zhongguo Zhong Yao Za Zhi ; (24): 3855-3861, 2018.
Article de Zh | WPRIM | ID: wpr-775406
Bibliothèque responsable: WPRO
ABSTRACT
To explore the effects of shading and the expression of key enzyme genes on the synthesis and accumulation of Panax japonicus var. major saponins, different shading treatments (0%, 30%,50%) of potted P. japonicus var. major were used as test materials, the expression of three key enzyme genes(CAS,DS,-AS) of leaves and rhizomes in different growth periods of P. japonicus var. major was determined by real-time quantitative PCR, the content of total saponins was determined by ultraviolet spectrophotometry. The results indicated that, in flowering stage, CAS,DS,-AS were highly expressed in the aerial parts of P. japonicus var. major, 30% shading treatment significantly inhibited the expression of CAS in leaves and promoted the expression of DS and -AS in stems, leaves and flowers, it was speculated that the main part of saponin synthesis was leaf in this stage. Both the expression levels of DS and -AS and changes in the content of total saponins in leaves showed a tendency of low-high-low throughout the growth cycle, correlation coefficient analysis showed that there was a positive correlation between them. Compared with control, the expression levels of DS and -AS and the content of total saponins were greatly enhanced under shading treatment, 30% shading treatment significantly promoted the accumulation of total saponins. Therefore, it is suggested that 30% shading treatment should be applied to the artificial cultivation of P. japonicus var. major, which is beneficial to the accumulation and quality improvement of saponins.
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Texte intégral: 1 Indice: WPRIM Sujet Principal: Effets des rayonnements / Saponines / Feuilles de plante / Régulation de l'expression des gènes végétaux / Rhizome / Génétique / Panax / Lumière langue: Zh Texte intégral: Zhongguo Zhong Yao Za Zhi Année: 2018 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Effets des rayonnements / Saponines / Feuilles de plante / Régulation de l'expression des gènes végétaux / Rhizome / Génétique / Panax / Lumière langue: Zh Texte intégral: Zhongguo Zhong Yao Za Zhi Année: 2018 Type: Article