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Inducing Effect of Decitabine on Apoptosis of KMS-18 Myeloma Cells and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1149-1153, 2019.
Article de Zh | WPRIM | ID: wpr-775750
Bibliothèque responsable: WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of decitabine on proliferation and apoptosis of multiple myeloma KMS-18 cells and its possible mechanism.@*METHODS@#CCK-8 was used to detect cell proliferation, flow cytometry was used to detect the changes of apoptosis, real-time quantitative PCR was used to detect the expression of P53 gene mRNA in myeloma KMS-18 cells, and MSP assay was used to detect the methylation status of P53 gene promoter.@*RESULTS@#The proliferation inhibition and apoptosis of KMS-18 cells significantly increased after treatment by decitabine (P<0.05). The expression of P53 mRNA increased in KMS-18 cells after treatment of decitabine (P<0.05). The methylation status of the P53 gene promoter in KMS-18 cells could be partially reversed by decitabine.@*CONCLUSION@#Decitabine can inhibit the proliferation of KMS-18 cells and induce their apoptosis, its mechanism ralates with partially reversing the methylation of P53 gene promoter in KMS-18 cells.
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Apoptose / Méthylation de l'ADN / Lignée cellulaire tumorale / Prolifération cellulaire / Décitabine Limites du sujet: Humans langue: Zh Texte intégral: Journal of Experimental Hematology Année: 2019 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Apoptose / Méthylation de l'ADN / Lignée cellulaire tumorale / Prolifération cellulaire / Décitabine Limites du sujet: Humans langue: Zh Texte intégral: Journal of Experimental Hematology Année: 2019 Type: Article