Changes in Metabolism and Function of Platelets Stored at Different Temperature / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 2003-2008, 2019.
Article
Dans Zh
| WPRIM
| ID: wpr-781504
Responsable en Bibliothèque :
WPRO
ABSTRACT
@#AAbstractObjective:
To compare and analyze the metabolic and functional changes in platelets stored at 4 ℃ and ones stored at 22 ℃ with agitation so as to provide an experimental basis for the cryopreservation technology of platelets.@*METHODS@#Samples were collected from platelets stored at 4 ℃ in 2, 4, 6, 11, 15 and 21 days, and from ones stored at 22 ℃ with agitation during the same days, the metabolism indicators and thromboelastogram (TEM) were analysed.@*RESULTS@#In metabolism, there were no significant changes of pH, GLU,PCO2, PCO2 and MPV levels of platelets stored at 4 ℃ for <6 days (P>0.05), However, the Plt count decreased, the PDW and LDH level incrseased (P<0.05). At the same time, only MPV had no changes of platelets stored at 22 ℃ during above-mentioned same days (P>0.05), while the pH, PCO2, GLU, Plt all decreased, and PO2, LDH, PDW incrseased (P<0.05). There were significant changes about the pH value, PO2, Plt, MPV, LDH, GLU levels between the two kinds of stored platelets during the same storing period (P<0.05). The pH value and MPV of platelets stored at 4 ℃ were obviously lower than ones stored at 22 ℃, while GLU, PO2, LDH and Plt levels showed reverse changes (P<0.05). Meanwhile, the PCO2 of platelets stored at 4 ℃ not could be detected and the Plt count reduced rapidly from d15. In function, the MA level of platelets stored at 4 ℃ was slower than that of platelets stored at 22 ℃, that is, the MA level of platelets stored at 4 ℃ were higher than that of platelets stored at 22 ℃ during the same storeing period (P<0.05).@*CONCLUSION@#Platelets stored at 4 ℃ have much slower metabolism than ones stored at 22 ℃, and the aggregation is stronger of platelets stored at 4 ℃ than that of ones at 22 ℃ during the same conservation period.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Température
/
Plaquettes
/
Conservation de sang
/
Cryoconservation
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2019
Type:
Article