CD8+T cells in peripheral blood of patients with acute antituberculosis drug-induced liver injury on the expression and significance of CD28 / 上海预防医学

ABSTRACT

Objective ] To detect the percentage of CD8+CD2-8、CD8+CD2+8 cells in patients with antituberculosis drug-induced liver injury and investigate their expression on acute liver injury by antituberculosis drugs and clinical significance. [ Methods] The flow cytometry was used to detect the percentages of CD8+CD2-8 and CD8+CD2+8 in peripheral blood of 42 patients with antituberculosis drug-induced acute liver injury, and 232 normal liver function patients with antituberculosis treatment.The mean amount of T cells between the two groups were compared by t-test and when P <0 .05 the difference found in comparison was thought to be statistically significant. [ Results] The percentage of CD8+CD2-8 cells of antituberculosis drug-induced acute injury group was lower than that of antituberculosis with normal liver function group (12.2 ±7.7%and 23.6 ±10.4%) , and the difference was of statistical significance ( t=4.782,P<0.01).And the amount of CD8+CD2+8 cells in antituberculosis drug-induced acute injury group was higher than that in antituberculosis with normal liver function group ( 23.5 ±9.1% and 10.2 ± 6.7%), the difference being statistically significant (t=5.356,P<0.01) too. [Conclusion] The amount of CD8+CD2-8 cells is reduced in peripheral blood of patients with antituberculosis drug-induced acute injury, while the amount of CD8+CD2+8 cells is increased.It is held that CD28 expression on CD8+T cells can be used as a marker of immunological detection in antituberculosis drug-induced acute liver injury.