Comparison of anti-bacterial activity of three types of di-O-caffeoylquinic acids in Lonicera japonica flowers based on microcalorimetry / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 108-113, 2014.
Article
Dans En
| WPRIM
| ID: wpr-812302
Responsable en Bibliothèque :
WPRO
ABSTRACT
The anti-bacterial activities of three types of di-O-caffeoylquinic acids (diCQAs) in Lonicera japonica flowers, a traditional Chinese medicine (TCM), on Bacillus shigae growth were investigated and compared by microcalorimetry. The three types of diCQAs were 3, 4-di-O-caffeoylquinic acid (3, 4-diCQA), 3, 5-di-O-caffeoylquinic acid (3, 5-diCQA), and 4, 5-di-O-caffeoylquinic acid (4, 5-diCQA). Some qualitative and quantitative information of the effects of the three diCQAs on metabolic power-time curves, growth rate constant k, maximum heat-output power Pm, and the generation time tG, total heat output Qt, and growth inhibitory ratio I of B. shigae were calculated. In accordance with a thermo-kinetic model, the corresponding quantitative relationships of k, Pm, Qt, I and c were established. Also, the half-inhibitory concentrations of the drugs (IC50) were obtained by quantitative analysis. Based on the quantity-activity relationships and the IC50 values, the sequence of inhibitory activity was 3, 5-diCQA > 4, 5-diCQA > 3, 4-diCQA. The results illustrate the possibility that the caffeoyl ester group at C-5 is the principal group that has a higher affinity for the bacterial cell, and that the intramolecular distance of the two caffeoyl ester groups also has an important influence on the anti-bacterial activities of the diCQAs.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Acide quinique
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Bacillus
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Médicaments issus de plantes chinoises
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Chimie
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Acide chlorogénique
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Concentration inhibitrice 50
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Lonicera
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Fleurs
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Antibactériens
Type d'étude:
Qualitative_research
langue:
En
Texte intégral:
Chinese Journal of Natural Medicines (English Ed.)
Année:
2014
Type:
Article