Anti-neuro-inflammatory effects of Nardostachys chinensis in lipopolysaccharide-and lipoteichoic acid-stimulated microglial cells / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 343-353, 2016.
Article
de En
| WPRIM
| ID: wpr-812605
Bibliothèque responsable:
WPRO
ABSTRACT
Excessive microglial cell activation is related to the progression of chronic neuro-inflammatory disorders. Heme oxygenase-1 (HO-1) expression mediated by the NFE2-related factor (Nrf-2) pathway is a key regulator of neuro-inflammation. Nardostachys chinensis is used as an anti-malarial, anti-nociceptive, and neurotrophic treatment in traditional Asian medicines. In the present study, we examined the effects of an ethyl acetate extract of N. chinensis (EN) on the anti-neuro-inflammatory effects mediated by HO-1 up-regulation in Salmonella lipopolysaccharide (LPS)- or Staphylococcus aureus lipoteichoic acid (LTA)-stimulated BV2 microglial cells. Our results indicated that EN suppressed pro-inflammatory cytokine production and induced HO-1 transcription and translation through Nrf-2/antioxidant response element (ARE) signaling. EN markedly inhibited LPS- and LTA-induced activation of nuclear factor-kappa B (NF-κB) as well as phosphorylation of mitogen-activated protein kinases (MAPKs) and signal transducer and activator of transcription (STAT). Furthermore, EN protected hippocampal HT22 cells from indirect neuronal toxicity mediated by LPS- and LTA-treated microglial cells. These results suggested that EN impairs LPS- and LTA-induced neuro-inflammatory responses in microglial cells and confers protection against indirect neuronal damage to HT22 cells. In conclusion, our findings indicate that EN could be used as a natural anti-neuro-inflammatory and neuroprotective agent.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
/
Acides teichoïques
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Extraits de plantes
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Lignée cellulaire
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Chimie
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Lipopolysaccharides
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Facteur de transcription NF-kappa B
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Microglie
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Neuroprotecteurs
/
Mitogen-Activated Protein Kinases
Limites du sujet:
Humans
langue:
En
Texte intégral:
Chinese Journal of Natural Medicines (English Ed.)
Année:
2016
Type:
Article