Assessment of the effects of Radix bupleuri and vinegar-baked Radix bupleuri on cytochrome 450 activity by a six-drug cocktail approach / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 302-308, 2013.
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| ID: wpr-812688
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ABSTRACT
AIM@#To assess the effects of Radix Bupleuri and vinegar-baked Radix Bupleuri on cytochrome 450 activity of rats.@*METHODS@#Six probe drugs (caffeine, midazolam, dextromethorphan, tolbutamide, omeprazole, chlorzoxazone) were simultaneously given to rats after different dosing of Radix Bupleuri or vinegar-baked Radix Bupleuri for seven days. The plasma concentrations of the six probes were measured by high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-MS/MS) and their corresponding pharmacokinetic parameters were calculated.@*RESULTS@#The AUC and T1/2 of midazolam, dextromethorphan and chlorzoxazone decreased significantly (P 0.05) from that of controlled rats, however, treatment of Radix Bupleuri decreased tolbutamide T1/2. The pharmacokinetics of caffeine in all Radix Bupleuri or vinegar-baked Radix Bupleuri-treated rats showed no statistically significant difference (P > 0.05) from that of controlled rats.@*CONCLUSION@#The Radix Bupleuri and vinegar-baked Radix Bupleuri have different effects on the CYP2C9 and CYP2C19. Radix Bupleuri and vinegar-baked Radix Bupleuri have strong induction effects on the CYP2E1, CYP2D6 and CYP3A4, however, have no impact on CYP1A2. The reason of different therapeutic effects of Radix Bupleuri and vinegar-baked Radix Bupleuri extract may be the different effects of Radix Bupleuri and vinegar-baked Radix Bupleuri on the CYP2C9 and CYP2C19.
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
Spectrométrie de masse
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Médicaments issus de plantes chinoises
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Pharmacocinétique
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Cinétique
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Chimie
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Chimie pharmaceutique
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Rat Sprague-Dawley
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Bupleurum
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Cytochrome P-450 enzyme system
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Antienzymes
Limites du sujet:
Animals
langue:
En
Texte intégral:
Chinese Journal of Natural Medicines (English Ed.)
Année:
2013
Type:
Article