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Expression characteristics of the Daxx gene in the mouse testis during spermatogenesis / 中华男科学杂志
Zhonghua nankexue ; Zhonghua nankexue;(12): 103-109, 2017.
Article de Zh | WPRIM | ID: wpr-812802
Bibliothèque responsable: WPRO
ABSTRACT
Objective@#To investigate the expression characteristic of the Daxx gene in the mouse testis and its role in spermatogenesis.@*METHODS@#Real-time PCR, Western blot and immunofluorescence were used in examining the expression characteristics of DAXX in the testis tissue from wild-type, Sertoli cell-specific androgen receptor knockout (SCARKO) and androgen receptor knockout (ARKO) mice at different postnatal weeks .@*RESULTS@#The Daxx gene was highly expressed in the testis tissue and mainly in the nuclei of the wild-type mice at 4 postnatal weeks. Compared with the wild-type, the ARKO mice showed a markedly decreased expression of DAXX (0.299±0.026), which displayed a polar distribution in the spermatogenic cells (0.853±0.058) and exhibited no significant difference in the SCARKO mice (1.000±0.015).@*CONCLUSIONS@#The Daxx gene expression is the highest in the middle-stage development of the mouse testis, significantly decreased in ARKO mice as compared with the wild-type, and its location influenced by specific AR knockout in Sertoli cells. DAXX may be involved in the regulation of spermatogenesis in mice.
Sujet(s)
Mots clés
Texte intégral: 1 Indice: WPRIM Sujet Principal: Cellules de Sertoli / Spermatogenèse / Testicule / Protéines nucléaires / Protéines de transport / Récepteurs aux androgènes / Expression des gènes / Noyau de la cellule / Souris knockout / Chaperons moléculaires Limites du sujet: Animals langue: Zh Texte intégral: Zhonghua nankexue Année: 2017 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Cellules de Sertoli / Spermatogenèse / Testicule / Protéines nucléaires / Protéines de transport / Récepteurs aux androgènes / Expression des gènes / Noyau de la cellule / Souris knockout / Chaperons moléculaires Limites du sujet: Animals langue: Zh Texte intégral: Zhonghua nankexue Année: 2017 Type: Article